BRYAN ANDREW LEONARD
BRYAN ANDREW LEONARD
5642 Little Ben Circle Apt. E
Columbus, Ohio 43231
(614) 818-0875
EDUCATION
Ohio State University, Major: Microbiology, M.S. March 1994

Kentucky State University, Major: Biology Education, B.S. May 1991
EXPERIENCE
Hilliard Davidson High School (August 1996-Present)

High School Biology teacher. In charge of teaching both lecture and laboratory exercises to honors and college prep Biology students.

Laboratory Research: Pathology and Pharmacy (June 1998-August 1998, June 1999-August 1999)

Ohio State University Medical/Science Research Initiative (MRI). Conducted kidney transplant research and quantified Alkaline Phosphotase presence in blood sample from patients with autoimmune disease. Studied regional chemotherapy techniques.

Mount Carmel College of Nursing (Jan. 1996-July 1996)

Instructor of Microbiology. Responsible for lecture and laboratory courses for nursing students.

Graduate Teaching Assistant (GTA) (Sept. 1993 June 1994)

Ohio State University GTA for undergraduate, graduate courses in Microbiology. Responsible for teaching laboratorycourses in animal cell tissue culture, clinical microbiology for dental students, and general microbiology.

Laboratory Research in Microbiology/Immunology (March 1992-June 1994)

Ohio State University Graduate program in Microbiology. Studied the antimicrobial mechanisms of macrophages to control and kill the intracellular bacterium, Brucella abortus. Performed infection experiments with transformed and nontransformed macrophages. Extensive animal tissue culture work.

Laboratory Research in Microbiology/Molecular Biology (June-Sept. 1991)

Ohio State University Graduate program in Microbiology. Induced site specific and random mutagenesis on genes to determine the effects of these mutations on Carbon dioxide fixation by photosynthetic bacteria. Genetic engineering, PCR and DNA cloning.

Laboratory Research in Toxicology (Sept. 1987-May 1991)

Kentucky State University Minority Biomedical Research Support (MBRS) Student Laboratory Assistant. Induction of mutations and sister chromatid exchange by metal compounds in chromosomes of culture CHO cells. Used cytological techniques for scoring chromosome aberration and sister chromatid exchange frequencies.

Laboratory Research in Medical Microbiology and Immunology (June-August 1990)

University of Wisconsin-Madison, Undergraduate Summer Research Program. Candidiasis in Germfree SCID mice. Cultured Candida albicans from G.I. tract and systemic organs from mice for quantitative analysis. Performed histopathology to determine if organs were colonized and infected over time. Study was done to determine if both B and T cells were required for protective immunity against C. albicans.

Laboratory Research in Experimental Pathology (June-August 1989)

University of Medicine and Dentistry of New Jersey at Newark, Undergraduate Summer Research Program. Worked on two projects: 1) Interferon alpha production by leukocytes when stimulated by HSV-1 and Sendai virus. 2) Natural killer cell assays. Measure Cr 51 release from human fibroblast cells infected with HSV-1.
PUBLICATIONS/PRESENTATIONS
Harris, K., T.S. Kochhar, B. Leonard, and W. Moody. (1988) Induction of Sister Chromatid Exchange by Metal Compounds, 16th NIH-MBRS Symposium, Los Angeles, CA.

Leonard, B., W. Howard, W., T.S. Kochhar, and W. Moody. (1990) Effect of Metal Ions in Causing Chromosome Changes in Cultured CHO Cells. Proceeding of International Symposium on Genetics Health and Disease. Amritsar, India.

Leonard, B., and T.S. Kochhar. (1990) Effect of Metal Ions in causing Chromosome Changes in Cultured CHO Cells., 4th Annual Black College Students High Tech Expo (In conjunction with 1990 NAFEO Conference). Washington,

D.C. Balish, E., J. Jensen, T Warner, J. Brekke, and B. Leonard. (1993) Mucosal and disseminated candidiasis in gnotobiotic SCID mice. Journal of Medical and Veterinary Mycology, 31: 1-12.

Jiang, X., B. Leonard, R. Benson, and C. Baldwin. (1993) Macrophage control of Brucella abortus: role of reactive oxygen intermediates and nitric oxide. Cell. Immunol. 151: 1-11.

Leonard, B., and C. Baldwin, (1993). The role of siderophores in the survival of Brucella abortus in macrophages. 46th Annual Meeting. Brucellosis Research Conference. Chicago, Illonois.

Leonard, BA, I. Lopez-Goni, and C. Baldwin. (1997) Brucella abortus siderophore 2,3-dihydroxybenzoic acid portects brucellae from killing by macrophages. Veterinarian Research 28:87-92.