Brad Harrub publication
Brad Harrub publication - from Web of Science
Title: Cryptic expression of the 70-kDa heat shock protein, hsp72, in gerbil hippocampus after transient ischemia

Author(s): Harrub JB, Nowak TS

Source: NEUROCHEMICAL RESEARCH 23 (5): 703-708 MAY 1998

Document Type: Article

Language: English

Cited References: 45, Times Cited: 8

Abstract: The 70 kDa heat shock protein, hsp72, is known to be induced following transient global ischemia in brain, as detected by immunocytochemistry and in situ hybridization techniques. However, while hsp72 mRNA is expressed rapidly following postischemic recirculation, immunocytochemistry fails to detect hsp72 protein for many hours after such insults, even in cell populations that readily express Fos and other proteins encoded by ischemia-induced mRNAs. In the present study, hsp72 expression in gerbil hippocampus was compared by immunocytochemistry and immunoblot methods at several intervals following 10 min ischemia. As established in previous studies, hsp72 immunoreactivity remained undetectable in postischemic neurons at 6 h following such insults. In contrast, immunoblots of dissected gerbil hippocampus demonstrated nearly maximal accumulation of hsp72 at this time point. These results indicate that the protein is present, but cryptic to detection in perfusion-fixed sections, during early recirculation. The constitutively expressed heat shock cognate protein, hsc70, did not show significant changes in level or distribution by either method, except for a decrease in CA1 staining at 48 h. These results confirm that hsp72 rapidly accumulates to high levels in postischemic hippocampus, and suggest that further studies of its subcellular localization during this interval may offer insight into its functional role as a component of the stress response in neurons after such insults.

Author Keywords: gerbil; ischemia; heat shock; Hsp72; Hsc70

KeyWords Plus: MESSENGER-RNA INDUCTION; STRESS PROTEIN; BRAIN POLYSOMES; RAT-BRAIN; INDUCED HYPERTHERMIA; ENERGY-METABOLISM; CEREBRAL-ISCHEMIA; TEMPORAL PROFILE; GLOBAL-ISCHEMIA; L-DOPA

Addresses: Nowak TS (reprint author), Univ Tennessee, Dept Neurol, 855 Monroe Ave,Link 415, Memphis, TN 38163 USA
Univ Tennessee, Dept Neurol, Memphis, TN 38163 USA
Univ Tennessee, Dept Anat & Neurobiol, Memphis, TN 38163 USA

E-mail Addresses: tnowak@utmem1.utmem.edu

Publisher: PLENUM PUBL CORP, 233 SPRING ST, NEW YORK, NY 10013 USA

Subject Category: BIOCHEMISTRY & MOLECULAR BIOLOGY; NEUROSCIENCES

IDS Number: ZG369

ISSN: 0364-3190